tracing wt braf codon 600 (Pyrosequencing Inc)
Structured Review

Tracing Wt Braf Codon 600, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tracing wt braf codon 600/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Frequency and Spectrum of BRAF Mutations in a Retrospective, Single-Institution Study of 1112 Cases of Melanoma"
Article Title: Frequency and Spectrum of BRAF Mutations in a Retrospective, Single-Institution Study of 1112 Cases of Melanoma
Journal: The Journal of Molecular Diagnostics : JMD
doi: 10.1016/j.jmoldx.2012.10.002
Figure Legend Snippet: Profile of BRAF Mutations in 499 Cutaneous Melanomas, Including Primary and Metastatic Acral and Nonacral Lesions
Techniques Used:
Figure Legend Snippet: Detection of BRAF c.1799_1800delinsAA (the so-called p.V600E2) in melanoma by pyrosequencing and Sanger sequencing. A: Pyrosequencing tracing shows the WT BRAF codon 600 as GTG. B: In mutant BRAF codon 600, a prominent peak represents A after the first G (white arrow), and a reduced peak represents the third base G (black arrow). C: The precise variant sequence (GAA) is confirmed by Sanger sequencing, which demonstrates a heterozygous pattern of T/A G/A at the second and third base positions of codon 600 (arrows).
Techniques Used: Sequencing, Mutagenesis, Variant Assay